Publications
TEM SEGA USA is proud to display here various Published Scientific Researches in fields including toxicology, oncology, medical imaging and neurosciences, all conducted with our Gas Anesthesia Stations recognized for their very low isoflurane consumption.
The Ultra Compact Tem Sega USA Gas Anesthesia Stations made for
small animals like rodents (mouse, rat, guinea pig, rabbit, ferret
and marmoset) are a highly accurate contribution to
scientific research in numerous specific fields.
Neuroscience
Age-dependent deficits of auditory brainstem responses in juvenile Neurexin1a knockout rats
S Marashli, P Janz, RL Redondo - Scientific Reports, 2024 - nature.com
...During the measurement, animals were under isofluorance-based anesthesia; induction at 5%, and maintenance at 2.5% (MiniHUB-V3, Tem Sega, FR). Body temperature was maintained...
Scalable batch fabrication of ultrathin flexible neural probes using a bioresorbable silk layer
C Cointe, A Laborde, LG Nowak - DN Arvanitis... - Microsystems &..., 2022 - nature.com
...With 3% vaporized isufluorane (TEM SEGA'S MINIHUB V3. Pessac). Once the mouse was placed on the surgical platform, anesthesia was maintained with isufluorane (1.5% v/v in 0 2). ...
PACAP and a novel stable analog protect rat brain from ischemia: Insight into the mechanisms of action
A Dedja, T Seaborn, S Bourgault - O Touzani... - Peptide, 2021 - Elsevier
...Anesthesia was maintained during the operation using 1.5% isufluorane in oxygen provided via a mask (Tem Sega,... Pessac). Before anesthesia was discontinued, wound was sutured and ...
Oncology
7aaRGD-a novel SPP1/integrin signaling-blocking peptide reverses immunosuppression and improves anti-PD-1 immunotherapy outcomes in experimental gliomas
A Ellert-Miklaszewska, P Pilanc, K Poleszak - AJ Roura... - 2024 - researchsquare.com
...) via an iso urane vaporizer (Temsega, Tabletop Anesthesia Station). Before starting the surgical procedure and during the surgery, the depth of anesthesia was verifed. The choice of ...
Tumor Necrosis Factor Superfamily 14 drives the depletion of tissue-resident alveolar macrophages upon influenza A virus infection enabling the establishment of secondary pneumococcal pneumonia
M Christina... - 2023 - Faculty of Medicine of the Justus Liebig University Giessen
...More specifically, oxygen containing 4% isoflurane was supplied under constant flow into the induction chamber of an anesthesia device [TEM SEGA, (FR)]. ...
Tumour-derived CSF2/granulocyte macrophage colony stimulating factor controls myeloid cell accumulation and progression of gliomas
M Sielska , P Przanowski , M Pasierbińska , K Wojnicki... - Cellular and Molecular Biology 2020 -nature.com/bjc
...Ten weeks old male mice were anesthetised with isoflurane inhalant anaesthesia (4–5% induction, 1–2% maintenance, 21% oxygen) using Isoflurane vaporiser (Temsega, Tabletop Anesthesia Station), and ...
Virology
Die Proteinkinase R abhängige Virulenz des influenza A Virus wird durch zwei Aminosäurestellen (R35 und R46) im NS1-Protein beeinflusst
J Bespalowa - 2022 - jlupub.ub.uni-giessen.de
...wenigen Sekunden war die sedierung erfolgt un die maus konnte auf den intubationstisch (Braintree Scientific) verlagert werden, wo die Narkose durch eine Nasenmaske (Temsega)...
Multifaceted activation of STING axis upon Nipah and Measles virus-induced syncytia formation
L Amurri, C Dumont, R Pelissier, O Reynard... - 2024 - researchsquare.com
...All mice were inoculated intraperitoneally with 10 6 TCID50 NIV-Mal under inhalational anesthesia with isufluorane (isufluorane: ISOFLUTEK 1000 mg/g, Laboratorios Karizoo S.A.; anesthesia station: MiniHUB-V3, TEM SEGA, France).
Toxicology
Chelation treatment by early inhalation of liquid aerosol DTPA for removing plutonium after rat lung contamination
L Miccoli, MF Ménétrier, P Laroche... - Radiation..., 2019 - meridian.allenpress.com
... 0.9% under light gaseous anesthesia. some treated rats ... microperforated vibrating membrane; Tem Sega, Pessac, France)... of the diaphragm under pentobarbital anesthesia (60 mg.kg -...
Ms1-Type Bioengineered Spider Silk Nanoparticles Do Not Exhibit Toxicity in An In Vivo Mouse Model
T Deptuch, A Florczak, A Lewandowska... - Nanomedicine..., 2021 - tandfonline.com
Medical Imaging
New oil-in-water magnetic emulsion as contrast agent for in vivo magnetic resonance imaging (MRI)
N Ahmed, C Jaafar-Maalej, MM Eissa... - Journal of ​..., 2013 - ingentaconnect.com
... The anesthesia. protocol was conducted with an approved system ( TEM Sega, Lormont, France). First, the rats were placed in an anesthesia induction box using a mixture of 3%...
Assessment of age modulated vascular inflammation in ApoE-/- mice by USPIO-enhanced magnetic resonance imaging
Monica Sigovan, Amine Bessaad, Hasan Alsaid, Eric Lancelot... - Investigative​..., 2010 - journals.lww.com
... % for the induction and 1% to maintain anesthesia ( TEM SEGA, Lormont, France) and kept under... At the end of the post contrast MRI, whereas still under anesthesia, mice were killed by...
Immunology
Basophils drive the resolution and promote wound healing in adult and aged mice
J Bex , V Peter , C Saji , L Chapart... - BioRxiv ​..., 2024 - biorxiv.org
... Animals were anesthetized using 3% Isoflurane in an anesthesia system 506 (MiniHub, TemSega) for both wounding and blood harvesting on live animals. ...
The effects of acid aspiration on tissue-resident macrophages in extra-pulmonary organs
M Langelage - Faculty of Medicine of the Justus Liebig University Giessen ​..., 2024 - jlupub.ub.uni-giessen.de/
... 2.2.1.2 HCl application. Mice were anesthetized with isoflurane and intubated using an anesthesia machine, the MiniHub V2.1 gas anesthesia workstation for small animals (TemSega (France).
Histology
Immunolabelling of Thin Slices of Mouse Descending Colon and Jejunum
J Bellis, I Duluc, JN Freund, JR De Mey - Bio-protocol, 2013 - bio-protocol.org
...Tem Sega evaporator apparatus for isofluorane anesthesia 3. Scissors 3 cm and ... Anesthesize the mouse using isufluorane inhalation with the help of the Tem Sega evaporator...
The Tem Sega USA CO2 Euthanasia Station, designed
for mice and rats, is a very reliable asset to the process
of scientific research in different specific fields.
Immunology
Basophils drive the resolution and promote wound healing in adult and aged mice
J Bex , V Peter , C Saji , L Chapart... - BioRxiv ​..., 2024 - biorxiv.org
... Euthanasia was always 507 performed in a controlled CO2 chamber (TemSega) ...
Isolation and enrichment of mouse splenic T cells for ex vivo and in vivo T cell receptor stimulation assays
C Grosjean, J Quessada, M Nozais ... - 2021 - sciencedirect.com
...For euthanasia with CO2, mice are placed in a hermetically sealed box, then we use an automatic CO2 euthanasia machine (TemSega) which allows a sequence of 3 phases according to a strict and secure protocol 1) ‘induction’ phase which lasts 1 min and corresponds to a progressive saturation in CO2; 2) ‘Euthanasia’ phase which lasts 2 min (100% CO2); 3) ’Emptying’ phase which lasts 2 min and corresponds to CO2 absorption.
PD-L1- and IL-4-expressing basophils promote pathogenic accumulation of T follicular helper cells in lupus
J Tchen, Q Simon, L Chapart, M K. Thaminy,... - Nature Communications, 2024 - nature.com
... All mice were euthanized by CO2 inhalation in a regulated chamber (TemSega, France). ...
Oncology
Regulatory T cells promote cancer immune-escape through integrin αvβ8-mediated TGF-β activation
A Lainé, O Labiad, H Hernandez-Vargas, S This,... - Nature Communications - 2021 - nature.com
... Animals were euthanized with euthanasia automate (Tem Sega) using 50–100% CO2. All animals were...
GSDMD is associated with survival in human breast cancer but does not impact anti-tumor immunity in a mouse breast cancer model
B Boersma, V Puddinu, A Huard, S Fauteux-Daniel, ... - Front. Immunol. - 2024 - Frontiersin.org
... Mice were sacrificed by CO2 euthanasia (Tem Sega automate) when the tumor surface reached 200 mm2. For mice bearing...
Functionalized silk spheres selectively and effectively deliver a cytotoxic drug to targeted cancer cells in vivo
A Florczak, T Deptuch, A Lewandowska, K Penderecka,... - Journal of Nanobiotechnology -2020 - springer.com
...mice were sacrificed by using a device designed for the gas euthanasia of small rodents (TEM-SEGA, Pessac, France), and the tumors and organs were excised for further analysis. ...
Toxicology
Characterization of a Family of Scorpion Toxins Modulating Ca2+-Activated Cl− Current in Vascular Myocytes
JL Morel,*,†, N Mokrzycki,†, G Lippens, ... - Toxins 2022 ​..., 2022 - mdpi.com
... from rats (150 to 250 g of body weight) which were euthanized in a CO2 chamber according to the protocol defined by the manufacturer (CO2 rack, Tem-Sega, Pessac, France) in a dedicated room.
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Ms1-Type Bioengineered Spider Silk Nanoparticles Do Not Exhibit Toxicity in An In Vivo Mouse Model
T Deptuch, A Florczak, A Lewandowska... - Nanomedicine..., 2021 - tandfonline.com
Vascular System
Tetrodotoxin Decreases the Contractility of Mesenteric Arteries, Revealing the Contribution of Voltage-Gated Na+ Channels in Vascular Tone Regulation
J Park, C Proux, W Ehanno, L Réthoré, ... - Mar. Drugs - 2023 - mdpi.com
... Animals were euthanized in a CO2 chamber (Tem Sega, Pessac, France) with a concentration gradient: a first phase was carried out by mixing air with CO2 for 1 min with a flow rate of 10 L/min, and death was induced by pure CO2 for 1 min with a flow rate of 10 L/min. ...
Veratridine Induces Vasorelaxation in Mouse Cecocolic Mesenteric Arteries
J Park, C Sahyoun, J Frangieh, L Réthoré, ... - Toxins 2024 - mdpi.com
... The mice were euthanized by CO2 inhalation in an automated chamber (Tem Sega, Pessac, France). ...
Virology
Multifaceted activation of STING axis upon Nipah and measles virus-induced syncytia formation
L Amurri, C Dumont, R Pelissier, O Reynard, ... - Plos Pathogens - 2022 - journals.plos.org
...Euthanasia with CO2 was conducted under inhalational anesthesia with isoflurane as well, ... (TEM SEGA, France). ...